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Despite facing significant external and internal challenges, Sambhaji’s leadership in defending the Maratha Empire from Mughal dominance and his military campaigns against various regional powers remain key elements of his legacy.
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The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction. This is useful for limiting the amplification only to mRNA. You can also exclude such primers if you want to amplify mRNA as well as the corresponding genomic DNA.
Primer-BLAST now offers direct links from genome assembly pages, so you can easily select the specificity database. Primer-BLAST also now accepts multiple target templates making it easy to design primers that can amplify several similar sequences such as all splice variants of gene or the same target (16S, COI) from different strains or species.
Learn how to use NCBI's Primer-BLAST in our virtual workshop! NCBI's Primer-BLAST combines the primer design features of the popular Primer3 package with a specificity check that uses nucleotide BLAST to search for target and non-target matches in a background database. Primer-BLAST primers are suitable for use in all PCR based molecular biology protocols including target identification ...
