Primer-BLAST now offers direct links from genome assembly pages, so you can easily select the specificity database. Primer-BLAST also now accepts multiple target templates making it easy to design primers that can amplify several similar sequences such as all splice variants of gene or the same target (16S, COI) from different strains or species.

Learn how to use NCBI's Primer-BLAST in our virtual workshop! NCBI's Primer-BLAST combines the primer design features of the popular Primer3 package with a specificity check that uses nucleotide BLAST to search for target and non-target matches in a background database. Primer-BLAST primers are suitable for use in all PCR based molecular biology protocols including target identification ...

To that end, this review serves as a primer for new operators. With ICE guidance, the conventional wisdom of right hepatic vein to right portal vein shunt placement has been challenged in favor of an individualized, in vivo ultrasound assessment of hepatic and portal vein anatomy and a priori selection of optimal needle path between the two ...

Primer-BLAST now has a โ€œ Primers common for a group of sequences โ€ submission tab that allows you to design primers for a group of highly similar sequences. For example, you may want test for expression of any transcript of gene rather than a specific splice variant, so you want to design primers to cover all transcript variants.