STRONGSVILLE, Ohio โ€“ PPG Commercial Coatings has introduced DELFLEETยฎ Evolution F4935, F4936 and F4937 HS Epoxy Primers, a new series of high-solids, 2.1 VOC primers with performance ...

The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction. This is useful for limiting the amplification only to mRNA. You can also exclude such primers if you want to amplify mRNA as well as the corresponding genomic DNA.

Primer-BLAST now offers direct links from genome assembly pages, so you can easily select the specificity database. Primer-BLAST also now accepts multiple target templates making it easy to design primers that can amplify several similar sequences such as all splice variants of gene or the same target (16S, COI) from different strains or species.

Primer-BLAST now has a โ€œ Primers common for a group of sequences โ€ submission tab that allows you to design primers for a group of highly similar sequences. For example, you may want test for expression of any transcript of gene rather than a specific splice variant, so you want to design primers to cover all transcript variants.

To that end, this review serves as a primer for new operators. With ICE guidance, the conventional wisdom of right hepatic vein to right portal vein shunt placement has been challenged in favor of an individualized, in vivo ultrasound assessment of hepatic and portal vein anatomy and a priori selection of optimal needle path between the two ...